Thermo Fisher Scientific Invitrogen Attune NXT Flow Cytometer (2021)
This Flow Cytometer was procured from a company that was shutting down their San Diego location. The unit was manufactured in 2021, purchased and installed in 2022, then used until April 2024. It is in excellent condition and is still under warranty, although Thermo Fisher said the manufacturer warranty is not transferrable.
This package includes:
Thermo Attune NxT Flow Cytometer AFC2
Desktop PC running Attune NxT Software (w/2 Licensed dongles, 2 monitors, mouse and keyboard)
Communication and power cables
Inside the Flow Cytometer:
1 Blue Excitation Laser 488nm - 4 colors with the following emission filters (nm):
530/30
574/26
695/40
780/60
Specs:
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um
Data Acquisition Speed: up to 35,000 events/second with 34 parameters
Maximum Electronic Speed: 65,000 events/second with all parameters
No-wash, no-lyse sample preparation workflow
Cycle time: 20-40 min for tube
Attune Flow Cytometers are compact benchtop cell-analyzers with hardware features that enable exceptionally fast sample flow rates, high clog resistance, and outstanding reliability. Attune instruments can be configured with up to 4 spatially-separated lasers and 14 fluorescence detectors. Acoustic focusing technology enables sample flow rates up to 1,000 µL/min, ~10x times faster than traditional cytometers, without compromising data integrity. Attune Cytometric Software makes the system easy-to-use and offers an optional module for 21 CFR part 11 compliance.
The Attune CytPix model includes a high-speed brightfield camera to capture images of individual cells as they pass through the flow cell. Automated image analysis software produces morphometric data integrated with standard fluorescence parameters. These features enable researchers to enhance data precision and accuracy, and gain novel insights into their sample biology.
Try our instrument configurator tool to design a system to fit your research needs.
Features common to all Attune flow cytometers
The Attune NxT and Attune CytPix flow cytometers share many of the same features. The Attune CytPix flow cytometer has all the same hardware and functionality as the Attune NxT, with the addition of a high-speed brightfield camera and automated image analysis software. Described below are the features common to both instruments, such as acoustic focusing for sensitivity and fast acquisition, optical design for flexibility and reliability, and clog resistant fluidics. Jump ahead to learn about the imaging and image analysis capabilities of the Attune CytPix.
Try our instrument configurator tool to design a system to fit your research needs.
Acoustic focusing for sensitive analysis
Attune Flow Cytometers combine ultrasonic waves like those used in medical imaging with hydrodynamic forces to precisely position cells into a single, focused line in the central axis. Enabling cells to be tightly focused at the point of laser interrogation allows the system to collect more photons, helping to ensure data quality regardless of the sample-to-sheath ratio.
Acoustic focusing
Acoustic focusing (left) precisely positions cells in tight alignment even at higher sample rates resulting in less signal variation and better data quality. Traditional hydrodynamic focusing (right) widens the sample stream core at high sample rates that results in signal variation and compromised data quality.
Fast sample acquisition speed
Acoustic focusing also lets your lab rapidly acquire high-quality data. Users can achieve sample throughput rates of 12.5 µL/min or 1,000 µL/min, up to 10 times faster than traditional hydrodynamic focusing systems and acquisition speeds of 35,000 events per second. This means processing all samples—including low-concentration and precious samples—more quickly and accurately with minimal loss in quality.
Clog resistant fluidics
To prevent clogging and allow for volumetric analysis, the Attune Flow Cytometers use a positive displacement syringe pump to control sample volume. The system can perform volumetric cell counts in a known volume (gated or total events) and can easily gate out dead cells to count live cells only in a live/dead analysis. Unlike traditional cytometers that operate at a maximum pressure of 15 PSI, Attune Flow Cytometers control pressure at 75 PSI, reducing the likelihood of cell-cell interactions and resulting in clog resistance.
Flexible optical design
Attune Flow Cytometers are configurable with up to 4 spatially separated lasers and 16 parameters. Spatial separation provides flexibility for multicolor panel design and streamlines compensation. The system offers superior speed with acquisition rates of up to 35,000 events per/second with high sensitivity to meet a range of research requirements.
High sensitivity distinguishes between dim signals and background, resulting in less variation and better signal separation. Fluorescent resolution coefficient variation is less than 3% for a single peak, and predicted MESF is ≤80 (FITC), ≤30 (PE), ≤70 (APC). Sensitivity in comparison to competitive systems is described below.
Please contact us for shipping quote. Local pickup in San Diego available.
This Flow Cytometer was procured from a company that was shutting down their San Diego location. The unit was manufactured in 2021, purchased and installed in 2022, then used until April 2024. It is in excellent condition and is still under warranty, although Thermo Fisher said the manufacturer warranty is not transferrable.
This package includes:
Thermo Attune NxT Flow Cytometer AFC2
Desktop PC running Attune NxT Software (w/2 Licensed dongles, 2 monitors, mouse and keyboard)
Communication and power cables
Inside the Flow Cytometer:
1 Blue Excitation Laser 488nm - 4 colors with the following emission filters (nm):
530/30
574/26
695/40
780/60
Specs:
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um
Data Acquisition Speed: up to 35,000 events/second with 34 parameters
Maximum Electronic Speed: 65,000 events/second with all parameters
No-wash, no-lyse sample preparation workflow
Cycle time: 20-40 min for tube
Attune Flow Cytometers are compact benchtop cell-analyzers with hardware features that enable exceptionally fast sample flow rates, high clog resistance, and outstanding reliability. Attune instruments can be configured with up to 4 spatially-separated lasers and 14 fluorescence detectors. Acoustic focusing technology enables sample flow rates up to 1,000 µL/min, ~10x times faster than traditional cytometers, without compromising data integrity. Attune Cytometric Software makes the system easy-to-use and offers an optional module for 21 CFR part 11 compliance.
The Attune CytPix model includes a high-speed brightfield camera to capture images of individual cells as they pass through the flow cell. Automated image analysis software produces morphometric data integrated with standard fluorescence parameters. These features enable researchers to enhance data precision and accuracy, and gain novel insights into their sample biology.
Try our instrument configurator tool to design a system to fit your research needs.
Features common to all Attune flow cytometers
The Attune NxT and Attune CytPix flow cytometers share many of the same features. The Attune CytPix flow cytometer has all the same hardware and functionality as the Attune NxT, with the addition of a high-speed brightfield camera and automated image analysis software. Described below are the features common to both instruments, such as acoustic focusing for sensitivity and fast acquisition, optical design for flexibility and reliability, and clog resistant fluidics. Jump ahead to learn about the imaging and image analysis capabilities of the Attune CytPix.
Try our instrument configurator tool to design a system to fit your research needs.
Acoustic focusing for sensitive analysis
Attune Flow Cytometers combine ultrasonic waves like those used in medical imaging with hydrodynamic forces to precisely position cells into a single, focused line in the central axis. Enabling cells to be tightly focused at the point of laser interrogation allows the system to collect more photons, helping to ensure data quality regardless of the sample-to-sheath ratio.
Acoustic focusing
Acoustic focusing (left) precisely positions cells in tight alignment even at higher sample rates resulting in less signal variation and better data quality. Traditional hydrodynamic focusing (right) widens the sample stream core at high sample rates that results in signal variation and compromised data quality.
Fast sample acquisition speed
Acoustic focusing also lets your lab rapidly acquire high-quality data. Users can achieve sample throughput rates of 12.5 µL/min or 1,000 µL/min, up to 10 times faster than traditional hydrodynamic focusing systems and acquisition speeds of 35,000 events per second. This means processing all samples—including low-concentration and precious samples—more quickly and accurately with minimal loss in quality.
Clog resistant fluidics
To prevent clogging and allow for volumetric analysis, the Attune Flow Cytometers use a positive displacement syringe pump to control sample volume. The system can perform volumetric cell counts in a known volume (gated or total events) and can easily gate out dead cells to count live cells only in a live/dead analysis. Unlike traditional cytometers that operate at a maximum pressure of 15 PSI, Attune Flow Cytometers control pressure at 75 PSI, reducing the likelihood of cell-cell interactions and resulting in clog resistance.
Flexible optical design
Attune Flow Cytometers are configurable with up to 4 spatially separated lasers and 16 parameters. Spatial separation provides flexibility for multicolor panel design and streamlines compensation. The system offers superior speed with acquisition rates of up to 35,000 events per/second with high sensitivity to meet a range of research requirements.
High sensitivity distinguishes between dim signals and background, resulting in less variation and better signal separation. Fluorescent resolution coefficient variation is less than 3% for a single peak, and predicted MESF is ≤80 (FITC), ≤30 (PE), ≤70 (APC). Sensitivity in comparison to competitive systems is described below.
Please contact us for shipping quote. Local pickup in San Diego available.
This Flow Cytometer was procured from a company that was shutting down their San Diego location. The unit was manufactured in 2021, purchased and installed in 2022, then used until April 2024. It is in excellent condition and is still under warranty, although Thermo Fisher said the manufacturer warranty is not transferrable.
This package includes:
Thermo Attune NxT Flow Cytometer AFC2
Desktop PC running Attune NxT Software (w/2 Licensed dongles, 2 monitors, mouse and keyboard)
Communication and power cables
Inside the Flow Cytometer:
1 Blue Excitation Laser 488nm - 4 colors with the following emission filters (nm):
530/30
574/26
695/40
780/60
Specs:
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um
Data Acquisition Speed: up to 35,000 events/second with 34 parameters
Maximum Electronic Speed: 65,000 events/second with all parameters
No-wash, no-lyse sample preparation workflow
Cycle time: 20-40 min for tube
Attune Flow Cytometers are compact benchtop cell-analyzers with hardware features that enable exceptionally fast sample flow rates, high clog resistance, and outstanding reliability. Attune instruments can be configured with up to 4 spatially-separated lasers and 14 fluorescence detectors. Acoustic focusing technology enables sample flow rates up to 1,000 µL/min, ~10x times faster than traditional cytometers, without compromising data integrity. Attune Cytometric Software makes the system easy-to-use and offers an optional module for 21 CFR part 11 compliance.
The Attune CytPix model includes a high-speed brightfield camera to capture images of individual cells as they pass through the flow cell. Automated image analysis software produces morphometric data integrated with standard fluorescence parameters. These features enable researchers to enhance data precision and accuracy, and gain novel insights into their sample biology.
Try our instrument configurator tool to design a system to fit your research needs.
Features common to all Attune flow cytometers
The Attune NxT and Attune CytPix flow cytometers share many of the same features. The Attune CytPix flow cytometer has all the same hardware and functionality as the Attune NxT, with the addition of a high-speed brightfield camera and automated image analysis software. Described below are the features common to both instruments, such as acoustic focusing for sensitivity and fast acquisition, optical design for flexibility and reliability, and clog resistant fluidics. Jump ahead to learn about the imaging and image analysis capabilities of the Attune CytPix.
Try our instrument configurator tool to design a system to fit your research needs.
Acoustic focusing for sensitive analysis
Attune Flow Cytometers combine ultrasonic waves like those used in medical imaging with hydrodynamic forces to precisely position cells into a single, focused line in the central axis. Enabling cells to be tightly focused at the point of laser interrogation allows the system to collect more photons, helping to ensure data quality regardless of the sample-to-sheath ratio.
Acoustic focusing
Acoustic focusing (left) precisely positions cells in tight alignment even at higher sample rates resulting in less signal variation and better data quality. Traditional hydrodynamic focusing (right) widens the sample stream core at high sample rates that results in signal variation and compromised data quality.
Fast sample acquisition speed
Acoustic focusing also lets your lab rapidly acquire high-quality data. Users can achieve sample throughput rates of 12.5 µL/min or 1,000 µL/min, up to 10 times faster than traditional hydrodynamic focusing systems and acquisition speeds of 35,000 events per second. This means processing all samples—including low-concentration and precious samples—more quickly and accurately with minimal loss in quality.
Clog resistant fluidics
To prevent clogging and allow for volumetric analysis, the Attune Flow Cytometers use a positive displacement syringe pump to control sample volume. The system can perform volumetric cell counts in a known volume (gated or total events) and can easily gate out dead cells to count live cells only in a live/dead analysis. Unlike traditional cytometers that operate at a maximum pressure of 15 PSI, Attune Flow Cytometers control pressure at 75 PSI, reducing the likelihood of cell-cell interactions and resulting in clog resistance.
Flexible optical design
Attune Flow Cytometers are configurable with up to 4 spatially separated lasers and 16 parameters. Spatial separation provides flexibility for multicolor panel design and streamlines compensation. The system offers superior speed with acquisition rates of up to 35,000 events per/second with high sensitivity to meet a range of research requirements.
High sensitivity distinguishes between dim signals and background, resulting in less variation and better signal separation. Fluorescent resolution coefficient variation is less than 3% for a single peak, and predicted MESF is ≤80 (FITC), ≤30 (PE), ≤70 (APC). Sensitivity in comparison to competitive systems is described below.
Please contact us for shipping quote. Local pickup in San Diego available.
